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ATCC
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Image Search Results
Journal: Microbial Genomics
Article Title: Comparative genomics of wild-type and laboratory-evolved biofilm-overproducing Deinococcus metallilatus strains
doi: 10.1099/mgen.0.000464
Figure Lengend Snippet: Growth and biofilm formation of WT and mutant strains. (a) Confocal laser scanning microscopy image analysis of biofilm. Biofilm was stained with the Film Tracer Ruby staining dye for 30 min, then observed in each strain at 24, 48, and 72 h based on two differential (left) and three differential (right) images. (b) Quantification of biofilm formation using the crystal violet-staining method. All biofilm assays were performed using five replications. (c) Examination of ECM components (eDNA, proteins, and saccharides). All components were evaluated in triplicate and measured at 24, 48, and 72 h. (d) Growth tests of the WT (grey line) and mutant (no. 5: pink line, no. 6: blue line) strains in 20 ml of TYG medium with agitation. The graphs show the average of duplicate experiments and the error bars indicate the standard deviation.
Article Snippet: The cells were initially washed twice with phosphate-buffered saline (PBS) and inoculated into a 50 ml flask containing 20 ml of
Techniques: Mutagenesis, Confocal Laser Scanning Microscopy, Staining, Standard Deviation
Journal: Microbial Genomics
Article Title: Comparative genomics of wild-type and laboratory-evolved biofilm-overproducing Deinococcus metallilatus strains
doi: 10.1099/mgen.0.000464
Figure Lengend Snippet: Evaluation of dead cell counts and evidence supporting cell death. (a) The relative expression of stress response genes in mutants was evaluated by comparison with that in the WT strain using qRT–PCR. All data are reported as the average of three replicates, and the error bars indicate the standard deviation. A t -test was performed between the WT and each mutant strain (* P <0.05; ** P <0.01). The black horizontal dotted line represents a onefold change in expression, which is the relative expression level of the WT strain. (b) Flow cytometric analysis of PI-stained (red) and non-stained (black) cells in TYG medium after 24 h of cultivation with agitation. The x - and y -axes represent PE-A (fluorescence intensity) and cell counts, respectively. (c) DIC microscopic image analysis. DNA and membranes (vesicles) were stained with the DAPI and FM4-64 dyes, respectively. The white arrows indicate predicted vesicles (1000× magnification). (d) Measurement of protease activity in the WT, no. 5 and no. 6 strains. The y -axis represents the concentration of tyrosine, which is a product of casein degradation. Samples were measured in duplicate, and the average is reported.
Article Snippet: The cells were initially washed twice with phosphate-buffered saline (PBS) and inoculated into a 50 ml flask containing 20 ml of
Techniques: Expressing, Comparison, Quantitative RT-PCR, Standard Deviation, Mutagenesis, Staining, Fluorescence, Activity Assay, Concentration Assay
Journal: Microbial Genomics
Article Title: Comparative genomics of wild-type and laboratory-evolved biofilm-overproducing Deinococcus metallilatus strains
doi: 10.1099/mgen.0.000464
Figure Lengend Snippet: Mortality and biofilm formation comparison between WT and mutant strains in media with glucose, acetate and glyoxylate supplementation without agitation. (a) Growth of WT and mutant strains in glucose-, acetate- and glyoxylate-supplemented TY medium without agitation. The grey, red and blue lines indicate the growth curves of the WT, no. 5 and no. 6 strains, respectively. All experiments were performed in triplicate, and each of the dots and error bars represents the average and standard deviation of triplicates, respectively. (b) Flow cytometric analysis of PI-stained (red) and non-stained (black) cells in TYG medium after 24 h of cultivation without agitation. The x - and y -axes represent PE-A (fluorescence intensity) and cell counts, respectively. (c) Quantification of biofilm formation in glucose-, acetate- and glyoxylate-supplemented TY medium at 24 h of culture. NaAc, sodium acetate; NaGox, sodium glyoxylate.
Article Snippet: The cells were initially washed twice with phosphate-buffered saline (PBS) and inoculated into a 50 ml flask containing 20 ml of
Techniques: Comparison, Mutagenesis, Standard Deviation, Staining, Fluorescence
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: Screening of Sarawak Biodiversity Centre (SBC) Actinobacteria Culture Collection for rubber degraders.
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques:
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: Microtetraspora sp. AC03309 morphology: ( a ) surface colony morphology on Yeast Malt Extract (ISP2) agar; ( b ) spore-forming structures viewed using light microscope at 750× magnification on Soil Extract agar (SEA); ( c ) sporophore branching from aerial mycelia viewed using Field Emission Scanning Electron Microscope (FESEM, Quattro Thermo Fisher Scientific, Lanham, MD, USA); ( d ) sporophore with four spores viewed using Field Emission Scanning Electron Microscope (FESEM, Quattro Thermo Fisher Scientific, Lanham, MD, USA).
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques: Light Microscopy, Microscopy
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: Cluster of Orthologous (COGs) for Microtetraspora sp. AC03309 analyzed using egg-NOG mapper v.2 . CELLULAR PROCESSES AND SIGNALING [D] Cell cycle control, cell division, chromosome partitioning, [M] Cell wall/membrane/envelope biogenesis, [N] Cell motility; [O] Post-translational modification, protein turnover, and chaperones, [T] Signal transduction mechanisms, [U] Intracellular trafficking, secretion, and vesicular transport, [V] Defense mechanisms [W] Extracellular structures, [Y] Nuclear structure, [Z] Cytoskeleton; INFORMATION STORAGE AND PROCESSING [A] RNA processing and modification, [B] Chromatin structure and dynamics, [J] Translation, ribosomal structure and biogenesis, [K] Transcription, [L] Replication, recombination and repair; METABOLISM [C] Energy production and conversion, [E] Amino acid transport and metabolism, [F] Nucleotide transport and metabolism, [G] Carbohydrate transport and metabolism, [H] Coenzyme transport and metabolism, [I] Lipid transport and metabolism, [P] Inorganic ion transport and metabolism, [Q] Secondary metabolites biosynthesis, transport, and catabolism; POORLY CHARACTERIZED [R] General function prediction only, [S] Function unknown.
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques: Modification, Transduction
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: Microtetraspora sp. AC03309 CRISPR array detected from CRISPRFinder server.
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques: CRISPR
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: Microtetraspora sp. AC03309 antibiotic resistant gene identified using CARD database.
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques:
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: Microtetraspora sp. AC03309 drug target gene identified using PATRIC server and DrugBank server.
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques:
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: Microtetraspora sp. AC03309 putative genes involved in sulfur assimilation annotated using RAST server.
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques: Binding Assay
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: Microtetraspora sp. AC03309 secondary metabolite biosynthetic gene clusters (BGCs) were predicted using AntiSMASH 6.0.
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques:
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: Location of lcp1 and lcp2 , and adjacent genes in Microtetraspora sp. AC03309 located on chromosome (contig 3): 1, Transcriptional regulator, TETR family; 2, Long chain fatty acid CoA ligase (EC 6.2.1.3); 3, Acetyl CoA dehydrogenase, short chain specific (EC 1.3.99.2); 4; Isoquinoline 1-oxidoreductase alpha subunit ( oxiA ); 5, Isoquinoline 1-oxidoreductase beta subunit ( oxiB ); 6, latex clearing protein 1 ( lcp1 ); 7, latex clearing protein 2 ( lcp2 ); 8, hypothetical protein; 9, Transcriptional regulator, TETR family; 10, MFS transporter; 11, hypothetical protein; 12, N-acetyltransferase; 13, pirin.
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques:
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: Micrographs of ( a ) control, rubber samples without microbial cultivation (A & D: fresh latex pieces; B & E: latex glove pieces; C & F: tyre granules); ( b , c ) rubber samples used as carbon source for cultivating Microtetraspora sp. AC03309 (A,B,C) and Dactylosporangium sp. AC04546 (D,E,F), incubated for 30 days, 28 °C at 180 rpm.
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques: Incubation
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: ATR-FTIR profile for fresh latex pieces as carbon source in MSM broth for 30 days, 28 °C, 180 rpm ( A ) Control ( B ) Dactylosporangium sp. AC04546 ( C ) Microtetraspora sp. AC03309.
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques:
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: ATR-FTIR profile for latex glove strips as carbon source in MSM broth for 30 days, 28 °C, 180 rpm ( A ) Control ( B ) Dactylosporangium sp. AC04546 ( C ) Microtetraspora sp. AC03309.
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques:
Journal: Polymers
Article Title: Rubber Degrading Strains: Microtetraspora and Dactylosporangium
doi: 10.3390/polym13203524
Figure Lengend Snippet: ATR-FTIR profile for tyre granules as carbon source in MSM broth for 30 days, 28 °C, 180 rpm ( A ) Control ( B ) Dactylosporangium sp. AC04546 ( C ) Microtetraspora sp. AC03309.
Article Snippet: Analysis between Microtetraspora sp. AC03309 and
Techniques: